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human bone morphogenetic protein 2  (R&D Systems)


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    R&D Systems human bone morphogenetic protein 2
    Human Bone Morphogenetic Protein 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+bone+morphogenetic+protein+2/Recombinant+Human%2FMouse%2FRat+BMP-2+(E%2E+coli-expressed)/pmc12954436-109-9-15
    Average 94 stars, based on 36 article reviews
    human bone morphogenetic protein 2 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    In Vitro:

    Article Title: Interleukin-34 orchestrates bone formation through its binding to Bone Morphogenic Proteins
    Article Snippet: .. In vitro experiments Reagents Recombinant human Macrophage-Colony Stimulating Factor (M-CSF), human interleukin-34 (IL-34), human M-CSF receptor (M-CSFR/CD115), human TGF-β1, human bone morphogenetic protein 2 (BMP-2), human bone morphogenetic protein 4 (BMP-4), human bone morphogenetic protein 7 (BMP-7), human Noggin, Activin RIIA receptor (ActRIIA), human Activin RIIB receptor (ActRIIB), human TRANCE (RANKL) and antibody anti-human M-CSFR, Anti-Phosho-M-CSFR (Y723) were obtained from R&D Systems (Abingdon, UK). .. Anti-human IL34 (BT-34) mouse IgG1 monoclonal antibody was produced by Diaclone (Besançon, France) under patent (Heymann D, Ségaliny A, Brion R. University of Nantes /Nantes Hospital/INSERM, “Anti-IL-34 antibodies”.

    Recombinant:

    Article Title: Interleukin-34 orchestrates bone formation through its binding to Bone Morphogenic Proteins
    Article Snippet: .. In vitro experiments Reagents Recombinant human Macrophage-Colony Stimulating Factor (M-CSF), human interleukin-34 (IL-34), human M-CSF receptor (M-CSFR/CD115), human TGF-β1, human bone morphogenetic protein 2 (BMP-2), human bone morphogenetic protein 4 (BMP-4), human bone morphogenetic protein 7 (BMP-7), human Noggin, Activin RIIA receptor (ActRIIA), human Activin RIIB receptor (ActRIIB), human TRANCE (RANKL) and antibody anti-human M-CSFR, Anti-Phosho-M-CSFR (Y723) were obtained from R&D Systems (Abingdon, UK). .. Anti-human IL34 (BT-34) mouse IgG1 monoclonal antibody was produced by Diaclone (Besançon, France) under patent (Heymann D, Ségaliny A, Brion R. University of Nantes /Nantes Hospital/INSERM, “Anti-IL-34 antibodies”.

    Article Title: Collagen and bone morphogenetic protein-2 functionalized hydroxyapatite scaffolds induce osteogenic differentiation in human adipose-derived stem cells.
    Article Snippet: .. The amount of recombinant human bone morphogenetic protein-2 (rhBMP-2) released at each selected time point (Day 1, 2, 3, 5, 7, 10, 12, 14, 18, and 21) was measured using a BMP-2 Quantikine ELISA Kit (R&D Systems, Inc.) by the solid phase sandwich ELISA following the manufacturer's protocols. .. Compressive strength was determined using a universal testing machine (R&B UNITECH-T, Korea).

    Article Title: Mechanosensitive Ion Channel PIEZO1 Suppresses BMP2 ‐Induced Ossification of the Annulus Fibrosus Cells
    Article Snippet: .. We induced osteogenic differentiation by treating cells with recombinant human bone morphogenetic protein 2 (BMP2, R&D Systems, Minneapolis, MN, Cat. #355‐BEC‐010) every 72 h. The control group received an equivalent volume of 4 mM HCl instead of BMP2. .. We assessed osteogenic differentiation using RT‐qPCR after 2 weeks, Alizarin Red staining after 3 weeks, and immunocytochemistry for phospho‐Smad1/5/9 (p‐Smad1/5/9, Cell Signaling, Cat. #13820).

    Article Title: Inducing Angiogenesis in the Nucleus Pulposus.
    Article Snippet: .. BMSCs from n = 20 male approximately 8-week-old (200–250 g) Lewis rats were expanded in monolayers, transduced 18–24 h prior to surgery with 105 particle units (pu)/cell with recombinant adenoviral (Ad) vectors encoding human bone morphogenetic protein-2 (AdBMP-2), human BMP-7 (AdBMP-7) and human VEFG165 (AdVEGF) transgenes, and a sample of the gene-programmed cells was assessed 3 days after transduction for transgene expression using ELISA (VEGF and BMP2 using kits from R&D Systems Inc., Minneapolis, MN, and BMP7 using a kit from Alpha Diagnostic International, San Antonio, TX, USA). ..

    Article Title: Interleukin-34 orchestrates bone formation through its binding to bone morphogenic proteins
    Article Snippet: Slices were fixed with PFA 1% 5 min, rinsed with PBS 1x and incubated in Xgal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) solution overnight at 37 °C. .. Recombinant human Macrophage-Colony Stimulating Factor (MCSF), human interleukin-34 (IL34), human M-CSF receptor (MCSFR/CD115), human TGF-β1, human bone morphogenetic protein 2 (BMP2), human bone morphogenetic protein 4 (BMP4), human bone morphogenetic protein 7 (BMP7), human Noggin, Activin RIIA receptor (ActRIIA), human Activin RIIB receptor (ActRIIB), human TRANCE (RANKL) and antibody anti-human M-CSFR, Anti-Phosho-M-CSFR (Y723) were obtained from R&D Systems (Abingdon, UK). .. Recombinant human Macrophage-Colony Stimulating Factor (MCSF), human interleukin-34 (IL34), human M-CSF receptor (MCSFR/CD115), human TGF-β1, human bone morphogenetic protein 2 (BMP2), human bone morphogenetic protein 4 (BMP4), human bone morphogenetic protein 7 (BMP7), human Noggin, Activin RIIA receptor (ActRIIA), human Activin RIIB receptor (ActRIIB), human TRANCE (RANKL) and antibody anti-human M-CSFR, Anti-Phosho-M-CSFR (Y723) were obtained from R&D Systems (Abingdon, UK).

    Article Title: Demineralized bone fibers and preparation thereof
    Article Snippet: .. Triplicate wells of each culture plate were designated as a positive control and exposed to 150 ng mL−1 recombinant human bone morphogenetic protein 2 (rhBMP-2; R&D Systems catalog no. 355-BM-050/CF) in low-serum medium in lieu of DBM; a further three wells of each culture plate were designated as a negative control and exposed to low-serum medium without DBM fibers or other additives. ..

    Article Title: Single-Step Fabrication of Core–Shell Microgels for the Controlled Release of rhBMP-2 and Simvastatin to Induce Osteogenesis
    Article Snippet: Dual delivery of bioactive molecules (drugs and growth factors) has been attempted to enhance multiple processes during tissue regeneration.. For bone tissue engineering, many attempts have been made to enhance osteogenesis coupled angiogenesis, which plays a major role during the bone regeneration process.. In this study, core−shell microgels were fabricated for controlled release of recombinant human bone morphogenetic protein 2 (rhBMP-2) and simvastatin from the core and shell, respectively.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Collagen and bone morphogenetic protein-2 functionalized hydroxyapatite scaffolds induce osteogenic differentiation in human adipose-derived stem cells.
    Article Snippet: .. The amount of recombinant human bone morphogenetic protein-2 (rhBMP-2) released at each selected time point (Day 1, 2, 3, 5, 7, 10, 12, 14, 18, and 21) was measured using a BMP-2 Quantikine ELISA Kit (R&D Systems, Inc.) by the solid phase sandwich ELISA following the manufacturer's protocols. .. Compressive strength was determined using a universal testing machine (R&B UNITECH-T, Korea).

    Article Title: Inducing Angiogenesis in the Nucleus Pulposus.
    Article Snippet: .. BMSCs from n = 20 male approximately 8-week-old (200–250 g) Lewis rats were expanded in monolayers, transduced 18–24 h prior to surgery with 105 particle units (pu)/cell with recombinant adenoviral (Ad) vectors encoding human bone morphogenetic protein-2 (AdBMP-2), human BMP-7 (AdBMP-7) and human VEFG165 (AdVEGF) transgenes, and a sample of the gene-programmed cells was assessed 3 days after transduction for transgene expression using ELISA (VEGF and BMP2 using kits from R&D Systems Inc., Minneapolis, MN, and BMP7 using a kit from Alpha Diagnostic International, San Antonio, TX, USA). ..

    Article Title: Single-Step Fabrication of Core–Shell Microgels for the Controlled Release of rhBMP-2 and Simvastatin to Induce Osteogenesis
    Article Snippet: Dual delivery of bioactive molecules (drugs and growth factors) has been attempted to enhance multiple processes during tissue regeneration.. For bone tissue engineering, many attempts have been made to enhance osteogenesis coupled angiogenesis, which plays a major role during the bone regeneration process.. In this study, core−shell microgels were fabricated for controlled release of recombinant human bone morphogenetic protein 2 (rhBMP-2) and simvastatin from the core and shell, respectively.

    Sandwich ELISA:

    Article Title: Collagen and bone morphogenetic protein-2 functionalized hydroxyapatite scaffolds induce osteogenic differentiation in human adipose-derived stem cells.
    Article Snippet: .. The amount of recombinant human bone morphogenetic protein-2 (rhBMP-2) released at each selected time point (Day 1, 2, 3, 5, 7, 10, 12, 14, 18, and 21) was measured using a BMP-2 Quantikine ELISA Kit (R&D Systems, Inc.) by the solid phase sandwich ELISA following the manufacturer's protocols. .. Compressive strength was determined using a universal testing machine (R&B UNITECH-T, Korea).

    Control:

    Article Title: Mechanosensitive Ion Channel PIEZO1 Suppresses BMP2 ‐Induced Ossification of the Annulus Fibrosus Cells
    Article Snippet: .. We induced osteogenic differentiation by treating cells with recombinant human bone morphogenetic protein 2 (BMP2, R&D Systems, Minneapolis, MN, Cat. #355‐BEC‐010) every 72 h. The control group received an equivalent volume of 4 mM HCl instead of BMP2. .. We assessed osteogenic differentiation using RT‐qPCR after 2 weeks, Alizarin Red staining after 3 weeks, and immunocytochemistry for phospho‐Smad1/5/9 (p‐Smad1/5/9, Cell Signaling, Cat. #13820).

    Transduction:

    Article Title: Inducing Angiogenesis in the Nucleus Pulposus.
    Article Snippet: .. BMSCs from n = 20 male approximately 8-week-old (200–250 g) Lewis rats were expanded in monolayers, transduced 18–24 h prior to surgery with 105 particle units (pu)/cell with recombinant adenoviral (Ad) vectors encoding human bone morphogenetic protein-2 (AdBMP-2), human BMP-7 (AdBMP-7) and human VEFG165 (AdVEGF) transgenes, and a sample of the gene-programmed cells was assessed 3 days after transduction for transgene expression using ELISA (VEGF and BMP2 using kits from R&D Systems Inc., Minneapolis, MN, and BMP7 using a kit from Alpha Diagnostic International, San Antonio, TX, USA). ..

    Expressing:

    Article Title: Inducing Angiogenesis in the Nucleus Pulposus.
    Article Snippet: .. BMSCs from n = 20 male approximately 8-week-old (200–250 g) Lewis rats were expanded in monolayers, transduced 18–24 h prior to surgery with 105 particle units (pu)/cell with recombinant adenoviral (Ad) vectors encoding human bone morphogenetic protein-2 (AdBMP-2), human BMP-7 (AdBMP-7) and human VEFG165 (AdVEGF) transgenes, and a sample of the gene-programmed cells was assessed 3 days after transduction for transgene expression using ELISA (VEGF and BMP2 using kits from R&D Systems Inc., Minneapolis, MN, and BMP7 using a kit from Alpha Diagnostic International, San Antonio, TX, USA). ..

    Positive Control:

    Article Title: Demineralized bone fibers and preparation thereof
    Article Snippet: .. Triplicate wells of each culture plate were designated as a positive control and exposed to 150 ng mL−1 recombinant human bone morphogenetic protein 2 (rhBMP-2; R&D Systems catalog no. 355-BM-050/CF) in low-serum medium in lieu of DBM; a further three wells of each culture plate were designated as a negative control and exposed to low-serum medium without DBM fibers or other additives. ..

    Negative Control:

    Article Title: Demineralized bone fibers and preparation thereof
    Article Snippet: .. Triplicate wells of each culture plate were designated as a positive control and exposed to 150 ng mL−1 recombinant human bone morphogenetic protein 2 (rhBMP-2; R&D Systems catalog no. 355-BM-050/CF) in low-serum medium in lieu of DBM; a further three wells of each culture plate were designated as a negative control and exposed to low-serum medium without DBM fibers or other additives. ..

    other:

    Article Title: Laminin 111 triggers cell quiescence and long-term survival by inducing IQGAP1-mediated cytosolic scaffolding of ERK and BAD inactivation
    Article Snippet: The following small compound inhibitors (amongst the best-reported inhibitors for each kinase ) were used: GDC-0879 (called BRAFi in this study, Tocris 4453) used at 2.5 μM, ZM 336372 (called CRAFi in this study, Santa Cruz sc-200639) used at 2 μM, PD0325901 (called MEKi in this study, Tocris 4192) used at 5 μM, SCH772984 (called ERKi in this study, Selleckhem S7101) used at 5 μM, Staurosporin (Sigma Aldrich 19-123-MG) used at 2 μM, Doxorubicin (called Dox in this study, LKT Labs D5794) used at 50 nM, hydrogen peroxide (H2O2, Sigma Aldrich H1009) used at 0.4 mM, Human Epidermal Growth Factor (EGF, Sigma Aldrich E9644) used at 50 ng/mL, Human Tumour Necrosis Factor alpha (TNFα, Millipore GF442) used at 20 ng/mL, Human Transforming Growth Factor beta1 (TGFβ, R&D 240-B-002/CF) used at 5 ng/mL, and Human Bone Morphogenetic protein 2 (BMP2, R&D 355-BEC) used at 10 ng/mL.



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    Image Search Results


    Representative images of immunohistochemical staining and histopathological assessment from study specimens. (a) At Week 4, Group II, increased neovascularization (asterisk) is observed between collagen fibre bundles (arrow) within the tendon tissue (H&E staining, ×100 magnification). (b) At Week 4, Group II, collagen fibres stained blue and vascular structures were clearly distinguishable (Masson's trichrome staining, ×100 magnification). (c) Strong cytoplasmic immunopositivity for BMP‐7 in multinucleated cell formations, Group III, staining observed in cell clusters at the tendon–bone interface at Week 8 (immunoperoxidase staining, ×400 magnification). (d) Positive immunopositivity for CD34 was observed in vascular endothelial cells within the tendon region at Week 4, Group II (immunoperoxidase staining, ×200 magnification). (e) Membranous TGF‐β1 staining in the tendon–muscle junction at Week 4, Group I, showing sparse immunopositivity (immunoperoxidase staining, ×400 magnification). (f) Cytoplasmic immunopositivity for BMP‐2 at the tendon–bone interface at Week 8, Group III (immunoperoxidase staining, ×400 magnification). BMP‐2, bone morphogenetic protein‐2; BMP‐7, bone morphogenetic protein‐7; TGF‐β1, transforming growth factor‐β1.

    Journal: Journal of Experimental Orthopaedics

    Article Title: Comparative effects of bone marrow stimulation and shockwave therapy on rotator cuff healing in chronic massive rotator cuff tears: A rat model study

    doi: 10.1002/jeo2.70622

    Figure Lengend Snippet: Representative images of immunohistochemical staining and histopathological assessment from study specimens. (a) At Week 4, Group II, increased neovascularization (asterisk) is observed between collagen fibre bundles (arrow) within the tendon tissue (H&E staining, ×100 magnification). (b) At Week 4, Group II, collagen fibres stained blue and vascular structures were clearly distinguishable (Masson's trichrome staining, ×100 magnification). (c) Strong cytoplasmic immunopositivity for BMP‐7 in multinucleated cell formations, Group III, staining observed in cell clusters at the tendon–bone interface at Week 8 (immunoperoxidase staining, ×400 magnification). (d) Positive immunopositivity for CD34 was observed in vascular endothelial cells within the tendon region at Week 4, Group II (immunoperoxidase staining, ×200 magnification). (e) Membranous TGF‐β1 staining in the tendon–muscle junction at Week 4, Group I, showing sparse immunopositivity (immunoperoxidase staining, ×400 magnification). (f) Cytoplasmic immunopositivity for BMP‐2 at the tendon–bone interface at Week 8, Group III (immunoperoxidase staining, ×400 magnification). BMP‐2, bone morphogenetic protein‐2; BMP‐7, bone morphogenetic protein‐7; TGF‐β1, transforming growth factor‐β1.

    Article Snippet: For the purpose of histopathological evaluation, hematoxylin–eosin (H&E) staining and Masson's trichrome staining were performed in accordance with standard protocols, and for immunohistochemical analysis, antibodies against bone morphogenetic protein‐2 (BMP‐2), bone morphogenetic protein‐7 (BMP‐7) and transforming growth factor‐β1 (TGF‐β1) were obtained from Invitrogen (Thermo Fisher Scientific) and CD34 from Dako (Glostrup).

    Techniques: Immunohistochemical staining, Staining, Immunoperoxidase Staining